Association between changes in handgrip strength and depression in Korean adults: a longitudinal panel study

Depression in older adults is a global socioeconomic burden. Identifying factors, such as physical activity or exercise that can help prevent depression is important. We aimed to investigate the relationship between changes in handgrip strength and the presence of depression using longitudinal, nationwide data of older Korean adults. Data from the Korean Longitudinal Study of Aging were used in this study. A total of 6783 participants who had undergone a handgrip strength test and completed the short-form Center for Epidemiologic Studies Depression Scale (CESD-10-D) questionnaire from 2006 to 2018 were included. General estimating equations were used to assess the temporal effect of the changes in handgrip strength on depression. A decrease in handgrip strength was associated with high CESD-10-D scores (β = 0.1889 in men, β = 0.1552 in women). As a continuous variable, handgrip strength was negatively correlated with CESD-10-D scores(β = − 0.0166 in men, β = − 0.0196 in women). Changes in the handgrip strength were associated with depressive symptoms in our longitudinal study. Those who experienced a decrease in handgrip strength had severe depressive symptoms compared to those with unchanged or increased handgrip strength. These findings can be used to guide general health policies for the prevention of depression.

www.nature.com/scientificreports/ study used handgrip strength, which can be measured instantly and easily, as a proxy for the muscle power status of a participant 20 . Further, changes in handgrip strength can also be measured regularly to determine the exercise status of participants in a given period [21][22][23] . This study aimed to investigate the relationship between changes in handgrip strength and depressive symptoms in a Korean adult population cohort selected from a panel study.

Methods
Study population and data. The data analyzed in this study were extracted from the Korean Longitudinal Study of Aging database (KLoSA). The KLoSA is a longitudinal panel survey of national representative samples of community-dwelling adults aged above 45 years and has been conducted every two years since 2006 24 . The baseline data were gathered in 2006, where 10,254 Korean adults were interviewed by trained interviewers. The survey collected information on family background, demographics, family, health, employment status, income, and assets and included questionnaires on subjective expectations and subjective quality of life. In 2018, the seventh wave was conducted, and the effective sample number was 6,136 from the original panels and 804 from the newly included panels. In this study, we used biannual survey data from 2006 to 2018, resulting in seven rounds of data. After removing data with missing values for the study variables, 6,793 participants (3,052 men and 3,731 women) were included in this study. The baseline characteristics of the included and excluded individuals are shown in Table S1. For statistical analysis, each change in handgrip strength from 2006 to 2018, rather than each participant, was treated as an individual case.
Measures. The short-form Center for Epidemiologic Studies Depression Scale (CESD-10-D) was used for measuring depressive symptoms. The validity of the Korean version of CESD-10-D for screening of depression is well established 25,26 . The participants were asked to answer 10 questions about their depressive condition using a binomial scoring system. The KLoSA provides a raw score by adding the scores of all the answers, and this score ranges from 0 to 10, with high scores indicating high severity of depression. We used a CESD-10-D cut-off score of 3 to determine the association between the change in handgrip strength and the presence of depressive symptoms .

Handgrip strength.
Handgrip strength was measured in kilograms using a handgrip dynamometer (Hand Grip Meter 6103, Tanita, Tokyo, Japan). The participants were asked to squeeze the dynamometer twice with each hand, and the highest value among the four trials was used in this study. The participants who refused to perform the test due to physical problems were excluded from the data analysis. To analyze the relationship between the change in handgrip strength and the presence of depressive symptoms, the changes in both domains over the previous year were recorded. The continuous variable of handgrip strength was categorized into two groups: (1) decreased and (2) same or increased; the analyses were conducted assuming the presence of continuous changes in the recorded values across the two groups.
Covariates. Demographic and health-related factors were included as covariates in the analysis. The following demographic characteristics were included: age, educational level, dwelling region, working status, household income, participation in social activities, and the number of cohabiting generations 27 . The following healthrelated factors were included: smoking/alcohol use status, number of chronic medical conditions, body mass index (BMI), and perceived health status. All the covariates were measured using survey questionnaires. The KLoSA provides the number of chronic medical conditions for each participant, including hypertension, diabetes, cancers, chronic lung diseases, liver disease, cardiac disease, cerebrovascular diseases, psychiatric disease, arthritis, prostate disease, dementia, and other chronic diseases. All multivariable models were controlled for all of the covariates unless stated otherwise.
Statistical analysis. All statistical analyses were performed separately for men and women to rule out the effect of sex on depression. Analysis of variance was used to compare the general characteristics of the groups 27 . A generalized estimating equation (GEE) model was used for regression analysis of CESD-10 scores, change in handgrip strength, and other covariates. CESD-10 score was included as the outcome variable, and other variables in Table 1 were included in the GEE model. We used normal distribution with the identity link function for continuous variables and binomial distribution and logit link function for binary outcome variables. The temporal variable was the wave, i.e., every 2 years, and person ID was used to identify repeated subjects using the unstructured working correlation matrix for the GEE model. The analysis was conducted twice: first, after dividing the change in handgrip strength into two groups, and second, after setting the change in handgrip strength as a continuous variable. The results are presented as regression coefficients (β) and standard errors. Subgroup analyses were performed to assess the interaction between handgrip strength change and other variables that were associated with the CESD-10 score further. We conducted subgroup analyses for age, working status, participation in social activities, number of chronic medical conditions, and perceived health status. All analyses were carried out using SAS software version 9.4 (SAS Institute, Cary, North Carolina, USA), and the results were considered statistically significant if the p-value was < 0.05 and very highly significant if the p-value was < 0.001.

Results
The baseline characteristics of the study population, stratified by sex, are presented in Table 1. A total of 6,793 participants (3,052 men and 3,731 women) were included in the analysis. Of the participants, 39.7% of the men and 45.3% of the women showed either no changes in handgrip in the first two waves or an increase in the second wave. In the unadjusted analysis, we found no significant difference in CESD-10-D scores between the two handgrip strength groups in both sexes. However, the other covariates such as age, educational level, region, working status, household income, participation in social activities, number of chronic medical conditions, BMI, and perceived health status significantly differed in CESD-10-D scores for both sexes. Table 2 shows the multiple regression analysis results for the CESD-10-D score and groups of change in handgrip after adjusting for the covariates. Compared to the same or increased handgrip strength group, the decreased handgrip strength group showed regression coefficients of 0.1889 in men and 0.1552 in women, which were highly significant at p < 0.0001. A decrease in handgrip strength was associated with an increase in CESD-10-D scores in both sexes. The results of the other covariates are shown in Table 2. Table 3 shows the results of multiple regression analysis between CESD-10-D score and handgrip strength change as continuous values using the same covariates from Table 2. The regression coefficients were − 0.0166 in men and − 0.0196 in women, with a high significance level of p < 0.0001. These results suggest that the change in handgrip strength was negatively associated with the CESD-10-D total score in both sexes. Table 4 shows that the decreased handgrip strength group showed a higher odds ratio (OR) for depression (OR = 1.18, 95% confidence interval [CI] 1.10-1.27 in men, OR = 1.09, 95% CI 1.02-1.16 in women) after dividing the participants into two groups using the CESD-10-D cut-off score of 3.
The results of the subgroup analysis are shown in Table S2. When we grouped the data by age, all age groups showed high CESD-10 scores when their handgrip strength had decreased, except the oldest male and female groups, which showed no significant relationship between handgrip change and depression.

Discussions
We found that a decrease in handgrip strength during the previous 2 years was associated with depressive symptoms in Korean adults. The participants whose handgrip strength decreased had reported higher CESD-10-D scores than those whose handgrip strength had remained the same or increased in the same period. Furthermore, we found that handgrip strength was negatively correlated with the CESD-10-D score in our study population.
The results of the present study are generally consistent with those of previous studies, namely that handgrip strength was associated with a high prevalence of depression or an increase in depressive symptoms. For example, one study found that weak handgrip strength was associated with a high odds ratio for depression in low-and middle-income countries. Although it was a cross-sectional study, the odds ratio of depression was 1.45 in the weak hand strength group, suggesting that handgrip strength was directly related to depressive symptomology 28 . Furthermore, a cross-sectional study involving community-dwelling adults in the USA also found that sarcopenia, denoted by decreased handgrip strength, was associated with the presence of depression 29 . Another cohort study conducted in Japan by Fukumori et al. reported that low baseline handgrip strength was associated with depressive symptoms 30 . Reduced handgrip strength was also associated with the development of depression after 1 year in a longitudinal analysis; this study suggested that a higher handgrip strength might prevent depression.
The mechanism underlying the association between handgrip strength and the presence of depressive symptoms has not yet been established. However, several hypotheses have suggested that exercise has antidepressant effects, giving us a clue about the association between muscle strength, a result of exercise, and depressive symptoms. One hypothesis is that exercise relieves depressive symptoms by reversing depression-induced atrophy of brain structures. Atrophy of brain structures such as the hippocampus, anterior cingulate cortex, and prefrontal cortex has been reported in patients with depression 31 . Importantly, several studies have shown that exercise increases the volume of the hippocampus, anterior accumbens, and prefrontal cortex, thus providing antidepressant effects [32][33][34] . Activated muscle or increased muscle mass might produce antidepressant effects by elevating the level of serotonin in the brain. In fact, since muscles consume branched-chain amino acids as their metabolism substrates, the serum concentration of single amino acids such as tryptophan increases. High levels www.nature.com/scientificreports/ www.nature.com/scientificreports/ of tryptophan in the serum and cerebrospinal fluid lead to increased levels of serotonin and dopamine, resulting in a reduction in depression 35,36 . Exercise has also been found to increase the levels of brain-derived neurotrophic factor (BDNF), which is known to regenerate as well as enhance the function of the hippocampus. Given that decreased levels of BDNF have been found to be related to depression in older patients, exercise may not only reverse but also prevent depressive symptoms by increasing BDNF levels 37 . These previous studies suggest that exercise might prevent depression, and since we can measure the exercise status via handgrip strength, it might be associated with depression in individuals. This study had some limitations. First, we used survey-based data and excluded non-responders; thus, the results might have been affected by this bias. Individuals with severe depression or severe sarcopenia might not have accurately responded to the survey or may not have been included; thus, our results might have been underestimated. Second, we could not determine the biological risk factors for depression since we used only a survey-based database. As several biological factors have been established as risk factors for depression in adults, future studies should analyze them in regression models 38 . Third, causation could not be determined because of the lack of a prospective design. We used the change in handgrip strength between two waves and analyzed its association with depressive symptoms in the subsequent wave to minimize the risk of reciprocal causation. However, depressive symptoms in the previous waves could have caused a decrease in handgrip strength. Thus, future studies using a prospective design including interventions of strengthening exercises are needed to establish the causal relationship between changes in handgrip strength and depressive symptomology.
Despite these limitations, our study has many strengths. We conducted the analysis with a relatively large sample size that represented the general adult population of South Korea and used a longitudinal design. These results can be applied to the general Korean population to establish health care policies or conduct future studies. Second, we used standardized tools to measure muscle strength. Handgrip strength is easily measurable and can be standardized across different groups and studies. Third, we used the change in handgrip strength rather than baseline strength; thus, the results could be analyzed as the exercise or physical activity status of the previous period. Finally, our results suggest that increasing handgrip strength by modifying lifestyle habits is a useful strategy for preventing depressive symptoms in adults.
In conclusion, this longitudinal, large-sized study showed that change in handgrip strength was associated with depressive symptoms in South Korea. A decrease in handgrip strength was associated with severe depressive symptoms compared to no change or increased handgrip strength. Future studies exploring the underlying mechanisms of this association as well as the preventive effects of increasing handgrip strength on depressive symptoms may provide valuable strategies for treating and preventing depressive symptoms.

Data availability
The datasets analyzed during the current study are available in the KLoSA repository, https:// survey. keis. or. kr/ eng/ klosa/ datab oard/ List. jsp. Table 3. Results of the generalized estimating equation analysis of handgrip strength change as a continuous variable and short-form Center for Epidemiologic Studies Depression Scale scores. All covariates in Table 2 were included in this analysis. S.E. standard error.  Table 4. Results of the GEE analysis of handgrip strength change and depression. All variables in Table 2 were included in the GEE model.